Mucin competition was carried out by pretreating rAAV with bovine submandibular gland mucin (Sigma) for 30 min at 20°C. AAV vectors, in the presence or absence of mucin, were added to Cos-7 cells in equal volumes of DMEM for 1 h at 37°C. Cells were rinsed twice with DMEM and incubated at 37°C. Two thousand …
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The internalized bacteria were estimated by flow cytometry, by measuring the mean fluorescence intensity (MFI) of the cells. The phagocytosis was analysed by flow cytometry or confocal microscopy. Images were assembled and analysed with the Leica Confocal software LCS Lite 2. In the event you liked this short article and you want to obtain more …
All Dogs Sports Park, located on the picturesque grounds of Sycamore Lane Kennels and Farm in Lodi CA.
(209) 334-1316
Jonnee@alldogssportspark.com
5111 W Sargent Rd
Lodi, CA 95242
