Science : Sciences biologiques
The internalized bacteria were estimated by flow cytometry, by measuring the mean fluorescence intensity (MFI) of the cells. The phagocytosis was analysed by flow cytometry or confocal microscopy. Images were assembled and analysed with the Leica Confocal software LCS Lite 2.6 (Leica Microsystem). Images were acquired with a TCS SP2 AOBS confocal microscope (Leica Microsystem, …
Neu5Ac (blue), 2,7-anhydro-Neu5Ac (orange), or XY intermediate (gray). B and C, superposition of the 1H,13C HSQC reference spectra of Neu5Ac (blue) and 2,7-anhydro-Neu5Ac (orange) and 1H,13C HSQC reaction mixture at 30 min (where the XY peak is observed to peak, gray). The comparison of the 2D 1H,13C HSQC spectra of the substrate (2,7-anhydro-Neu5Ac), the product …
Uncovering a Novel Molecular Mechanism for Scavenging Sialic Acids in Bacteria Read More »
Neuraminidases and other reagents were obtained from Sigma-Aldrich. To determine whether sialic acids play a role in endothelial barrier function, cells were treated with neuraminidases to hydrolyze sialic acid moieties. Two components of glycocalyx structure that may play a role in determining function in glycoproteins are 1) the terminal sugar residue(s) on the oligosaccharide chain(s) …
Effect of Sialic Acid Loss on Dendritic Cell Maturation Read More »
Treatment of isolated-perfused lungs with neuraminidase from Vibrio cholerae leads to pulmonary edema. A third key observation in our study is the pattern of alveolar edema observed when isolated-perfused lungs were treated with neuraminidase from Vibrio cholerae. A second key observation in our study is that terminally linked sialic acids are important contributors to endothelial …
7 Facts Everyone Should Know About Sialic Acid Manufacturer Read More »
Some point out it has some flavor, not too unhealthy, and is a great addition to their morning coffee, whereas others say that it’s bitter and can upset the stomach. In addition they say the burst of energy is very refreshing and the supplement is the best for vitality and stamina. Who is AG1 greatest …
If you Wish To Be A Winner, Change Your Shiitake Mushroom Extract Powder Philosophy Now! Read More »
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